RNA
Part:BBa_K5099014:Design
Designed by: Isabelle Guo Group: iGEM24_McGill (2024-09-12)
+_tracrRNA_CasX
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
T7 promoter addition on dsDNA template was considered.
Source
Native cleavage locus of CasX enzyme. Sequence sourced from "CasX enzymes comprise a distinct family of RNA-guided genome editors", (Liu et al, 2019)